|
KMID : 1094720140190040605
|
|
Biotechnology and Bioprocess Engineering
2014 Volume.19 No. 4 p.605 ~ p.612
|
|
|
Molecular cloning and characterization of a novel glucansucrase from Leuconostoc mesenteroides subsp. mesenteroides LM34
|
|
Kang Hee-Kyoung
Nguyen Thi Thanh Hanh
Jeong Ha-Na
Park Min-Eon
Kim Do-Man
|
|
|
Abstract
|
|
|
|
Leuconostoc mesenteroides LM34 was isolated from kimchi, a traditional fermented Korean food. L. mesenteroides LM34 produced extracellular glucansucrase (DSRLM34), which is responsible for the synthesis of soluble glucan using sucrose. The DSRLM34 gene consists of a 4,503 bp open reading frame (ORF) and encodes an enzyme of 1,500 amino acids with an apparent molecular mass of 165 kDa. The deduced amino-acid sequence showed the highest amino-acid sequence identity (98%) to that of glucansucrase of Lactobacillus lactis. The gene was over-expressed in Escherichia coli strain and the recombinant enzyme (rDSRLM34) was purified. Both DSRLM34 and rDSRLM34 synthesized glucan mainly containing α-1, 6 glucosidic linkage and branched α-1, 3 glucosidic linkages. The enzyme exhibited optimum activity at 30°C and pH 5.0. DSRLM34 has promising potential as a thickening agent in sucrose-supplemented milk.
|
|
|
KEYWORD
|
|
|
Leuconostoc mesenteroides, glucansucrase, dextran, kimchi
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|